真菌多糖AbEXP1-a的结构及其免疫调节活性研究

Study on Chemical Structure and Immunomodulating Activity of Fungal Polysaccharide AbEXP 1-a

采用酸水解法、红外光谱法和核磁共振法对真菌多糖AbEXP1-a的结构进行了研究,并对该多糖的免疫调节活性进行了动物实验检测。结果表明,多糖AbEXP1-a分子由葡萄糖和甘露糖以3:1的比例构成。推测其主链由3个葡萄糖分子通过β-(1→6)糖苷键连在一起,再以α-(1→6)或β-(1→6)糖苷键与甘露糖连接而构成。多糖AbEXP1-a对小鼠非特异性免疫、体液免疫和细胞免疫均有明显的促进作用,可提高小鼠迟发性变态反应,即多糖AbEXP1-a可以全面提高机体的免疫功能。其最适作用剂量为10mg/kg体重。

The chemical structure of fungal polysaccharide AbEXP 1-a was studied through acid hydrolysis, IR spectroscopy and NMR spectroscopy. And its immunomodulating activity was tested through animal experiments. The results are as follow： AbEXP 1-a is consisted of glucose and mannose in ratio of 3：1. Its backbone was deduced that consisted of 3 glucoses combined by β-（1→6） glycosidic linkage and a meanose linking to them through α-（1→6） or β-（1→6） glycosidic linkage. AbEXP 1-a was found to be capabte of promoting non-specific immunity, humoral immunity and cellular immunity of mice. Besides, it also could promote delayed type hypersensitivity in mice. All that means that AbEXP la could enhance the immunity of organisms comprehensively and the appropriate dosage is 10 mg/kg body weight.