摘要
革兰氏染色(Gram stain),是细菌学中一个经常使用和十分重要的方法,自从1884年微生物学家Gram氏发明著名的革兰氏染色法以后,100多年来虽然经过后来学者的几次改进,但都仍然沿用着Gram氏原来的四步法,基本原理也没有改变。最近Allen氏对Ziehl-Neelsen抗酸菌染色法的改进,是一个良好的启示,使我们开始了革兰氏染色三步法的研究并取得了成功。现将我们建立的革兰氏染色三步法与质量控制报告如下。 1 材料和方法 1.1 结晶紫染色液 甲液:结晶紫2g;95％乙醇20ml。 乙液:草酸铵0.8g;蒸馏水80ml。 甲乙二液先分别溶解,然后混合在一起,过滤除去残渣后装入滴瓶中备用。
A simplified Gram's staining method is described. The decolorization step of 95% alcohol in the original Gram's stain was omitted, and sub-stituted by a counter stain prepared with 0.4% fuchsin in alcohol which acts as both decolorization and counter staining in the simplified Gram staining method. Under quality control, a lot of different bacteria including Gram positive and negative organisms were stained with the simplified Gram staining method. The result was comparable to the original Gram stain. The simplified Gram staining method is a time-saving and practical method that could be used as a routine technic in the bacteriological laboratories.
出处
《微生物学报》
CAS
CSCD
北大核心
1996年第1期76-78,共3页
Acta Microbiologica Sinica
