库拉索芦荟对实验性口腔鳞癌发生影响的初步研究

Chemopreventive Effect of A.Vera(Chinensis) on 4-NQO Induced Oral Carcinogenesis in Rats

目的:观察库拉索芦荟对4-NQO诱导的大鼠口腔粘膜鳞癌发生的影响.方法:51只Wistar大鼠,随机分为5组,除对照组(9只,不处理)和丙二醇组(9只,仅涂丙二醇)外,肿瘤组(9只)和高低剂量预防组(各12只)均用0.5%4-NQO涂擦腭粘膜至12周.预防组动物同时灌喂高或低剂量芦荟12周.20周时所有动物同时处死,切取腭粘膜进行病理学评价和免疫组化PCNA检测.结果:肿瘤组癌变率为88.9%.高低剂量预防组癌变率分别为16.7%和25%.统计分析表明,高低剂量预防组癌变率明显低于肿瘤组(P<0.01),高低剂量预防组间无差异(P>0.05);PCNA阳性指数高低剂量预防组明显低于肿瘤组(P<0.05).结论:库拉索芦荟对实验性口腔粘膜鳞癌的发生具有一定的预防作用.

Objective： The present study was undertaken to investigate the inhibitory effect of Aloe vera （chinensis） on 4 - nitroquinoline 1 - oxide （4 - NQO） - induced oral carcinogenesis in Wistar rats. Methods： 51 Wistar rats （6-7 weeks） were randomly divided into 5 groups after breeding for 1 week routinely. The 0.5 % 4 - NQO in propylene glycol was applied in all groups to treat their palate except an untreated control group, and propylene glycol alone in treated group for 12 weeks （3 times/week）. The rats in preventive group were also fed A.Vera （chinensis） in high dosage （ 1.8 g/kg/d ） or low dosage （ 0.3 g/kg/d ） for 12 weeks, then switched to the basal diets for 8 weeks. The rats were necropsied at the 20th week. The inci-dences of palate neoplasms and cell proliferation activity estimated by PCNA label index were compared among groups. Results： The incidences of palate neoplasms in4 - NQO alone was 88.9 %. The frequency of palatal carcinoma in preventive group feeding A. Vera （chinensis） at high or low dosage was significantly reduced： In high dosage 16.7% （P〈0.01） or low dosage 25% （P〈0.01）. Dietary administration of the aloe signifi- cantly decreased the PCNA label index of rat palatal mucosa , compared with those given 4 - NQO alone （P 〈 0.05）. Conclusion： Aloe at the 2 different doses has the chemopreventive effect on oral mucosa carcinogenesis.