γ射线与咖啡因复合处理对大麦的诱变效应

THE EFFECTS OF DAMAGE AND MUTAGENESIS OF GAMMA RAYS COMBINED WITH CAFFEINE ON BARLEY

用五种不同剂量的γ射线和５ｍｍｏｌ／Ｌ咖啡因对大麦种子进行单独处理和γ射线与咖啡因间的复合处理，结果表明：辐照Ｍ１代的细胞学损伤及植株损伤指标均与辐照剂量呈直线相关，且相关系数达显著或极显著水平；Ｍ２代苗期的叶绿素突变率和性状变异率均与辐照剂量呈指数函数关系，相关系数也均达显著或极显著水平。咖啡因本身对大麦不具诱变性，但以它作辐射后处理．不仅能加剧Ｍ１代细胞学损伤及植株损伤，而且能提高Ｍ２代叶绿素突变率和农艺性状变异率，表明咖啡因作辐射后处理是提高诱变效率的有效措施。

Barley seeds were treated with five different doses of gamma rays and 5 mmol/L caffeine respectively and the two together in the present study. The significant linear relationship between chromosome damage and plant damage of M1 and radiation dose were found in the present study. The remarkable expontial function relation was also found between the mutation rate of chlorophyll and characters and radiation dose. Caffeine does not have mutagentic effects when it is used as a mutagen itself only on barley. However, it not only can enhance damage on both chromosomes and plants of M1,but does the same for the mutation frequencies of chlorophyll and agronomic traints in M2 when it is used after radiation. This indicates that caffeine is an effective measure which can increase mutagenetic effects used after radiation.