摘要
通过单向水平变性聚丙烯酰胺凝胶等电聚焦（ＩＥＦ）、十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）和双向电泳（ＩＥＦ×ＳＤＳ—ＰＡＧＥ）分析了红苋Ｒ１０４种子谷蛋白的亚基组成，亚基分子量和等电点分布。谷蛋白的双向电泳图谱可分辨出１００多个亚基成分．其主要亚基为：５４ｋＤ（ｐＩ７．１５）；３３ｋＤ（ｐＩ５．８２）；３１ｋＤ（ｐＩ６．９２；ｐＩ６．７０；ｐＩ６．６５）；２２ｋＤ（ｐＩ８．３４）；２０ｋＤ（ｐＩ６．９２；ｐＩ６５６）；１８ｋＤ（ｐＩ６．９２；ｐＩ７３５；ｐＩ７．７２；ｐＩ８．０５）。另外，还对ＩＥＦ方法进行了讨论。
Isoelectric focusing(IEF), sodium dodecyl sulfate-polyaerylacvy laminde gel electrophoresis(SDS-PAGE) and two-dimensional electrophoresis (LEF* SDS-PAGE) were emploied to analyse the glutelin of grain amaranth (A. cruentus) for its patterns of subunits composition, molecular weight(MW) and isoelectric point (pI)of each subunits.The resultant electrophoregrom shown that the major subunits included 54kD (pI 7. 15),33kD(pI5. 82), 31kD (pI6. 92, pI6. 70, pI6. 56), 22kD (pI8. 34 ), 20kD (pI6. 92, pI 6. 56) and 18kD (pI6. 92, pI7. 35, pI7. 72, pI 8. 05). Moreover, the procedure of IEF were also discussed.
出处
《西北植物学报》
CAS
CSCD
北大核心
1996年第2期174-179,共6页
Acta Botanica Boreali-Occidentalia Sinica
关键词
苋科
红苋
籽粒苋
谷蛋白
电泳分析
Amaranth cruentus
glutelin
seed protein
two-dimensional gel electrophoresis
