感染流行性出血热病毒的正常人骨髓细胞膜脂和酶生物活性的研究

Study on Biologic Activity for Membrane and Enzyme of Normal Bone Marrow Cells Infected with Epidemic Hemorrhagic Fever Virus

采用疏水性荧光探针ＤＰＨ（１，６，－苯基－１，３；５－已三烯）标记感染了流行性出血热病毒的正常人骨髓细胞的细胞膜，根据荧光偏振值的变化推导出膜脂流动性，同时通过细胞组化染色定量方法，测定细胞内酸性磷酸酶（ＡＣＰ）和琥珀酸脱氢酶（ＳＤＨ）的活性。动态观察１、６、２４、７２ｈ培养后的淋巴细胞、单核细胞、中性粒细胞，发现感染后细胞膜脂的流动性明显降低，并随培养时间的延长而明显，和正常对照组比较有显著性差异（Ｐ＜０．０５～０．０１）。通过组化染色和显微分光光度计单细胞扫描定量认为：感染细胞在培养开始的７２ｈ内ＡＣＰ、ＳＤＨ升高，１２０ｈ后明显下降，和正常相比亦有显著差异（Ｐ＜０．０５），这与病毒侵犯和细胞机能的受损程度有关。

sing DPH fluorescence probe,the membrane of normal bone marrow cells infected withepidemic hemorrhagic fever virus(EHFV)was labeled.The membrane lipid fluidity could becounted according to change of value of fluorescence polarization.At the same time,using cyto-histochemical staining quantitative method,the cellular ACP and SDH were detected.The resultsshowed:(1)The Lymphocyte,monocyte and granulocyte were observed dynamically after cul-tured 1,6,24,72 hours.The membrane lipid fluidity of infected cells appeared obviously low-There were significant differences statistically(p<0.05-0.01)(2)By cytohisto-chemical stain-ing and microspectrophotometer quantitating,before cultured 72 hours,ACP and SDH in infectedcells raised.However,after 120 hours,their values were decreased Also,there were significant(P<0.05).It could be related with virus invasion and damage degree of cellular function.