以珍汕97B和9311为背景的导入系构建及其筛选鉴定

Development and Selection of Introgression Lines with the Identical Genetic Background of Varieties Zhenshan 97B and 9311

以我国主要推广的优良杂交稻亲本(珍汕97B和9311)为受体亲本,以从国际合作网络所征集的品种资源150多份为供体材料,进行了大规模杂交、3￣4次回交,1￣2次自交,培育珍汕97B和9311为背景的近等基因导入系近5000多份。通过对导入系材料进行品质、耐盐、耐旱、磷高效、氮高效利用等性状的筛选鉴定,获得了大量目标性状的导入系。通过对品质性状导入系的基因型分析,定位了影响粒型的15个染色体区段(或基因)。同时,构建了一套来源于粳稻日本晴的单片段导入系群体。创建的近等基因导入系(群体)为目标基因的发掘、新品种的培育提供重要的材料基础。

We initiated a large backcross breeding program for last 5 years, using two elite parents （Zhenshan 97B and 9311） of a number of rice hybrids widely cultivated in China as recipient, and 150 plus diverse accessions obtained from the International Cooperation Network of Rice Molecular Breeding Project as donors. A significant progress has been made in this program, of which about 5000 introgression lines （IL） with the identi- cal genetic background of the recurrent parents were generated through 3-4 times backcrossing and 1-2 selfing. A large number of target lines with desirable traits such as good quality, phosphorus and nitrogen use efficiency, drought tolerance and salinity tolerance, were obtained after extensive screening and selection of these ILs under different stresses. Fifteen chromosomal segments （genes） conferring grain shape were detected via using a number of selective introgression lines with good appearance quality. Meanwhile, a set of near-isogenic introgresslon lines each containing a single marker-defined introgressed segment from a japonica variety Nipponbare were developed. The created ILs not only provide a basis of breeding materials for developing new rice cultivars, but also unique genetic stocks for discovery of genes of interest.