水稻高世代回交导入系耐盐性的遗传研究

Genetic Analyses of Salt Tolerance in an Advanced Backcross Population of Rice

水稻是一种对盐敏感的作物,盐分是限制水稻产量的主要因子之一。本研究利用广泛推广种植的杂交籼稻的亲本珍汕97(ZS97)和测序粳稻品种日本晴(Nipponbare)杂交、回交构建的导入系,对水稻的耐盐性进行遗传研究。用88份导入系作苗期盐胁迫(0.3%氯化钠)处理试验,发现6份导入系的耐盐性同受体亲本ZS97有显著差异,其中5份导入系耐盐,1份对盐敏感。图示基因型分析表明5份导入系中含有少数外源导入片段及耐盐相关的基因。

Rice （Oryza sativa L.） is an irrigated crop sensitive to salinity. High salinity is one of the major constraints to rice production. To detect the quantitative trait loci for salt tolerance in rice, a total of 88 introgression lines （IL） selected from an advanced backcross population that derived from a cross between ZhenshanB （ZS97, indica） and the sequenced japonica variety Nipponbare, were treated in sodium chloride solution （0.3%）. Six ILs showing significant difference from ZS97 in salt tolerance were detected. Of those, five lines increased salt tolerance and the other one with decreased tolerance. Graphical genotyping suggests that those lines with tolerance changes harbor single or few introgression segments with salt tolerant genes from Nipponbare.