炭凝集试验快速检测呼吸道合胞病毒的研究

Study of Carbon Aggl utinaion Test for the Rapid Detection of Respiratory Syncytial Virus

用炭凝集试验（ＣＡＴ）检测呼吸道合胞病毒（ＲＳＶ），结果表明该法是一种简便、快速、特异的诊断方法。用ＣＡＴ对１６株ＲＳＶ和８株其它病毒做试验，结果仅ＲＳＶ凝集，而其它病毒均阴性。用该法与细胞培养法检测８３份临床呼吸道感染幼儿鼻咽吸出物，结果ＣＡＴ法阳性率为６９．８８％（５３／８３），细胞培养法为３９．７５％（３３／８３），两者阳性检出率相差极显著。阻断试验证明ＣＡＴ是高度特异的。结果证明ＣＡＴ具有较高的敏感性与特异性，可用于临床ＲＳＶ标本的快速检测。

his study reports a simple,rapid and special diagnosis method of carbon agglutination test(CAT)to detect Respiratory Syncytial Virus(RSV).Sixteen specimens of RSV were seen agglu-tination but 8 specimens of other virus were negative.We compared CAT with tissus culture byusing nasopnaryngeal secretions from children with suspected RSV iofection.Of 83 specimens,53(69.88%)were positive by CAT,33(39.75%)were positive by virus culture,There were verysignificant difference in the positive rates beween CAT and culture Blocking test showed thatCAT has high specificity.We believe that CAT has a high sensitivity and specificity and it can beused for the rapid detection of clinical specimens of RSV.