EB病毒转化Hu－TLC－SCID小鼠脾细胞的实验研究

Transformation of Spleen Cells Prepared from Dengue Virus-2 Immunized Hu-TLC-SCID Mice

利用ＥＢ病毒转化可产生较高水平人ＩｇＧ和特异性抗２型登革病毒人抗体的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞，通过免疫组化、免疫荧光和ＰＣＲ法检测转化细胞的人Ｂ细胞表面标志、ＥＢ病毒抗原和ＥＢ病毒基因。结果表明，被转化的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞能继续产生抗２型登革病毒的特异性人抗体，并具有人Ｂ细胞的、ＳｍＩｇＧ标志及ＥＢ病毒潜伏膜蛋白－１（ＬＭＰ－１）基因，可表达ＬＭＰ－１和ＥＢ病毒核抗原（ＥＢＮＡ）。

he spleen cells isolated from SCID mice transplanted with human tonsillar lymphocytes(Hu- TLC-SCID)immunized with dengue virus 2 type(DV_2)in vitro and in vivo were trans-formed by Epstein-Barr virus(EBV).Specific human anti-DV_2 antibodies were detectable in thesupernatant of the transfomed spleen cells.The presence of human B lymphocyte markers(and SmIgG) as well as EBV antigens (LMP1 and BENA)and LMP1 gene fragment in the trans-formed spleen cells determined by immunoperoxidase staining immunofluorescence technique andPCR technique,indicated that the Hu-TLC-SCID mouse spleen cell has been successfully trans-foimed by EBV.The EBV-transformed Hu-TLC-SCID mouse spleen cell could be utilized forcontinous production of specific human anti-DV_2 antibodies in vitro.