摘要
对香蕉花叶病广东三个代表分离物的形态和生物学特征进行了较为全面的研究。在六种鉴别寄主上,斑驳类(MM)和断续条纹类(BS)分离物侵染较多的寄主种类诱导较为严重的症状,连续条纹类(CS)分离物侵染能力较弱。电镜观察表明:BS、CS和MM三个代表分离物的粒子呈球状、其粒子直径分别为22.5、24.3和27nm,纯化的病毒粒子电泳相对迁移率分别为0.72、0.56和0.51,在西葫芦上的增殖速度:不管在子叶或真叶,都是MM最快、BS次之、CS最慢。从西葫芦接种叶运转出接种叶的速度:MM和BS相似、CS明显较慢。在烟草上,三个分离物的运转速度和增殖速度以MM最快、BS次之、CS最慢;而且在烟草上随着接种后时间的延长,三个分离物除MM维持高浓度的时间较长外,BS和CS的都很短。在西葫芦和烟草上提纯产量,MM类最多、BS类次之、CS类最低。
he morphological and biological characters of three CMV isolates infecting banana fromGuangdong were studied.According to the different reactions in six kinds of host plants includingCucumis sativus,Nicotiana tabacum cv,HV38,Lycopersicon esculentum,Cucumis melover.saccharins,and sepecially Musa acuminata,the three isolates could be distinctly distinguished.The diameters of the virus particle of BS,CS and MM were 22.5, 24.3 and 27 nm,and the rela-tive electrophoretic mobilities of their particles were 0.72,0.56 and 0.51,respectively.The MMisolate multiplied and spreaded more rapidly with higher concentrations in Cucurbita pepo andNicotiana tabacum than the BS or the CS isolate,and in turn then BS isolate more rapidly thanthe CS isolate.
出处
《中国病毒学》
CAS
CSCD
1996年第2期149-156,共8页
Virologica Sinica
基金
广东省科学基金
高等学校博士点专项科研基金