侵染香蕉的黄瓜花叶病毒株系的形态和生物学特征

Morphological and Biological Characters of Cucumber Mosaic Virus Strains Infecting Banana

对香蕉花叶病广东三个代表分离物的形态和生物学特征进行了较为全面的研究。在六种鉴别寄主上，斑驳类（ＭＭ）和断续条纹类（ＢＳ）分离物侵染较多的寄主种类诱导较为严重的症状，连续条纹类（ＣＳ）分离物侵染能力较弱。电镜观察表明：ＢＳ、ＣＳ和ＭＭ三个代表分离物的粒子呈球状、其粒子直径分别为２２．５、２４．３和２７ｎｍ，纯化的病毒粒子电泳相对迁移率分别为０．７２、０．５６和０．５１，在西葫芦上的增殖速度：不管在子叶或真叶，都是ＭＭ最快、ＢＳ次之、ＣＳ最慢。从西葫芦接种叶运转出接种叶的速度：ＭＭ和ＢＳ相似、ＣＳ明显较慢。在烟草上，三个分离物的运转速度和增殖速度以ＭＭ最快、ＢＳ次之、ＣＳ最慢；而且在烟草上随着接种后时间的延长，三个分离物除ＭＭ维持高浓度的时间较长外，ＢＳ和ＣＳ的都很短。在西葫芦和烟草上提纯产量，ＭＭ类最多、ＢＳ类次之、ＣＳ类最低。

he morphological and biological characters of three CMV isolates infecting banana fromGuangdong were studied.According to the different reactions in six kinds of host plants includingCucumis sativus,Nicotiana tabacum cv,HV38,Lycopersicon esculentum,Cucumis melover.saccharins,and sepecially Musa acuminata,the three isolates could be distinctly distinguished.The diameters of the virus particle of BS,CS and MM were 22.5, 24.3 and 27 nm,and the rela-tive electrophoretic mobilities of their particles were 0.72,0.56 and 0.51,respectively.The MMisolate multiplied and spreaded more rapidly with higher concentrations in Cucurbita pepo andNicotiana tabacum than the BS or the CS isolate,and in turn then BS isolate more rapidly thanthe CS isolate.