大鼠小肠淤血再灌注损伤的实验研究

Experimental Study on Congestion-Reperfusion Injury to Small Intestine in Rats

目的研究淤血后再灌注对大鼠小肠的损伤作用及对肠组织丙二醛(MDA)含量和髓过氧化物酶(MPO)活性的影响。方法SD大鼠20只,雌雄各半,采用阻断门静脉60min开放60min的方法建立大鼠小肠淤血再灌注模型;于小肠淤血前、淤血60min和再灌注60min时分别取末段回肠标本,观察和比较各时间点小肠黏膜的损伤情况,同时测定肠组织中MDA含量利MPO活性的变化。结果①淤血前肠黏膜基本正常,淤血60min后肠黏膜山现损伤,但组织中MDA含量及MPO活性与淤血前相比无明显变化;②再灌注60min后肠黏膜损伤较淤血60min时进一步加重,且组织中MDA含量及MPO活性亦显著增高。结论肠道组织在一定时间淤血后血流再灌注情况下可出现淤血再灌注损伤;脂质过氧化损伤及中性粒细胞(PMN)浸润可能是参与肠组织淤血再灌注损伤的重要因素。

Objective To study the congestion-reperfusion injury, to small intestine and its effects on MDA and MPO in rats. Methods The model of congestion-reperfusion of intestine was established by means of the occlusion of portal vein for 60 minutes and then reflow for 60 minutes in 20 Sprague-Dawley rats. Ileum tissue was harvested respectively for histological examination and MDA and MPO determination at each time of before congestion,congestion for 60 minutes and reperfusion for 60 minutes. Results ①Intestinal mucosa was significantly injured after congestion for 60 minutes while that wasnt injured basically before congestion. MDA concentration and MPO activity in intestine tissue after congestion for 60 minutes were the same as those before congestion. ②After reperfusion for 60 minutes,the mucosa lesion was worser and the MDA concentration and MPO activity were markedly higher compared with those after congestion for 60 minutes. Conclusion Congestion-reperfusion injury happened to the intestine after the congestion of intestine for 60 minutes and reperfusion for 60 minutes in rats. It may be associated with the hyperoxidation of lipid and PMN infiltration in intestine.