钳夹损伤对周围神经脂质过氧化影响的实验研究

EXPERIMENTAL STUDY ON THE EFFECTS OF PEROXIDATION OF NEUROLIPID FOLLOWING CRUSHING INJURY OF PERIPHERAL NERVE/

周围神经损伤后的自由基变化研究极少，为了探索自由基在神经损伤及其再生过程中的变化规律，设计了本研究。用钳夹法制作大鼠坐骨神经损伤模型，测定对照组及实验组５个时间点（２，４，６，１１及２１天）坐骨神经脂质过氧化物丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）和超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）的含量，结果显示实验组５个时间点ＭＤＡ含量均极显著高于对照组，随时间延长呈渐升趋势；ＳＯＤ含量亦均极显著高于对照组，且随时间延长呈渐降趋势。研究提示，钳夹及线扎坐骨神经可引发产生大量的自由基，这对周围神经再生的影响尚不清楚，有待进一步研究。

In order to understand the change of free radicals in the course of injury and regeneration of nerve, the sciatic nerve of Wistar rat was crushed to, prepare the model of nerve injury and measured the content of Malondialdehyde (MDA) and superoxide dismutase (SOD) of the nerve. Thirty rats were used in this study. The sciatic nerve on one side was crushed, the contralateral sciatic nerve was served as control. According to the time of assessment (2,4,6,11,21 days after crushing), the rats were divided into 5 groups. The MDA concentration of the control was 19.65±0.27 and that of the crushing groups at different time were 21.25±0.36, 21.98±0.35, 22.77±0.38, 23.73±0.13, 23.92±0.44, respectively (nmol/100mg pro, x±s), while the SOD concentration of the control was 119.18±0.58 and that of the crushing groups at different time were 144.85±1.70, 136.14±1.71, 130.58±0.57, 126.41±0.98, 122.36±0.79, respectively (ug/mg pro, x±s), . In the experimental groups, all the MDA concentrations were markedly higher than that of the control (P<0.01, t test) and tended to increase with the time passing by. The SOD concentrations in the experimental groups were also higher than that of the control (P<0.01, t test) and tended to decrease with the time passing on. The study suggested that after crushing or ligation of the nerve, the free radicals would increase.