碱性成纤维细胞生长因子促进受创皮肤再生的实验研究

EXPERIMENTAL STUDY ON bFGF IN PROMOTING SKIN REGENERATION AFTER TRAUMA

成纤维细胞生长因子（ｆｉｂｒｏｂｌｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ＦＧＦ）有促进肉芽，组织生长作用，但对皮肤的修复与再生的作用研究尚少，为此设计了本实验研究。采用大鼠与小型猪背部创伤模型，在无菌条件下用手术刀在动物背部切割皮肤，形成面积约２．５ｃｍ２的圆形创面。分别用碱性成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ），６０Ｕ／ｃｍ２创面以及相同剂量的生理盐水处理创面，隔天换药一次。结果表明，ｂＦＧＦ具有显著促进上皮细胞生长、加速创面上皮化速率以及增加愈合组织抗张力强度等作用。ｂＦＧＦ显著促进受创皮肤的再生效应可能与它能直接作用于皮肤基底细胞上特异性ｂＦＧＦ受体有关。

Taking Wistar rats and pigs as models, the basic fibroblast growth factor (bFGF) was studied on wound healing. Five rats and five pigs were used in the experiment. Each rat had 2 round shaped wound (1.8cm in diameter) and each pig had 4 wounds of the same size as that of the rat. One wound of each rat and 2 wound of each pig were dressed with bFGF saline (60U/cm 2). While the other wounds of the rats and pigs were dressed with normal saline as control. The wounds treated with bFGF were completely covered with granulation tissue on the 7th day after injury, and in 14 days the wounds were almost completely covered by epithelium. The bFGF would enhance the growth of the epithelial cells, reepithelization of the wound and the tensile strength of the tissue. It was concluded that the bFGF could promote skin regeneration, which might be the direct action of bFGF on the bFGF receptors in the basic cells of skin.