心肌梗塞患者血管紧张素转换酶基因缺失多态性分析

DELETION POLYMORPHISM IN THE GENE FOR ANGIOTENSIN CONVERTING ENZYME IN PATIENTS WITH MYOCARDIAL INFARCTION

血管紧张素转换酶（ＡＣＥ）基因的多态性被认为与血浆ＡＣＥ浓度有关，同时也是欧洲人群心肌梗塞的独立的危险因子，尤其是那些一般被认为是心肌梗塞低危者。作者应用ＡＣＥ基因内含于１６多态区两侧的序列作为引物，用多聚酶链反应方法来检测ＡＣＥ基因多态性，对中国人中４０例心肌梗塞患者和４０例非冠心病患者进行了比较，结果表明心肌梗塞患者中ＤＤ基因型发生率（５０％）明显高于对照组（２０％）．在中国人中ＡＣＥ／ＤＤ基因型可能是心肌梗塞的重要危险因子。

Polymorphism in the angiotensin-converting enzyme(ACE) gene has been shown to correlate with circulating ACE concentrations,and also to be an independent risk factor for the development of myocardial infarction(MI) in European population,particularly in men thought to be at low risk by standard criteria. We determined the genotypes of 40 patients with myOcardial infarction and compared them with 40 control individuals without coronary artery disease in Chinese.Genotypes were determined by the polymerase chain-reaction with oligonucleotide primers flanking the polymorphic region in intron 16 of the ACE gene to amplify template DNA isolated from MI and control individuals.Here we reported that the DD genotype was significantly more frequent in patients with MI(50%)than in controls(20%).The ACE/DD genotype seems to be a potent risk factor of MI in Chinese.