人参皂甙对培养小鼠皮层神经细胞在糖氧剥离损伤中的保护作用

The protective effects of ginsenosides on cultured cortical neurons in oxygen-glucose deprivation in vitro

目的研究人参皂甙(GS)的三种单体成分GSRb1、GSRb3、GSRg1对离体培养的小鼠皮层神经细胞在糖氧剥离损伤中的保护作用。方法原代培养的小鼠胎鼠大脑皮层神经细胞分为正常对照组、糖氧剥离模型组、GSRb1、GSRb3、GSRg1干预组。利用糖氧剥离(OGD)建立皮层神经细胞缺血/再灌注损伤模型,分别用60μmol/L GSRb1、GSRb3、GSRg1干预后,再测定各组细胞培养上清液一氧化氮(NO2-/NO3-)、乳酸脱氢酶(LDH)、四唑盐(MTT)比色试验测定神经细胞活力,用流式细胞仪检测细胞凋亡率。结果GSRb1、GSRb3、GSRg1干预组与模型组比较,NO分泌量、LDH漏出量、细胞活力、细胞凋亡率均明显减少(P<0.01)。结论GSRb1、GSRb3、GSRg1对培养小鼠皮层神经细胞在糖氧剥离损伤中具有保护作用,其可能机制之一是通过拮抗兴奋性氨基酸毒性,减少NO分泌实现的。

Objective To investigate the protective effects of ginsenosides （GS）Rb1 , GSRb3, GSRg1 on cultured cortical neurons in oxygen-glucose deprivation （OGD） in vitro, Methods The mouse cortical neurons were cultured in vitro and divided randomly into the normal control group, OGD group,and groups of GSRb1, GSRb3, GSRg1. The cultured cortical neurons were pretreated by 60μmol/L GSRb1 ,GSRb3 or GSRg1 respectively. Contents of nitric oxide（NO） and LDH in all groups were measured after the ischemia-reperfusion model of cortical neurons was established. The cell viability was assayed by MTT, and the cell apoptosis rate was determined by cytoflowmetry. Results The contents of NO and LDH of OGD group significantly increased than those of the other groups（P〈0.01） ,and the cell viability significantly decreased in OGD group than that in the other groups（P〈0.01）. At the same time,the cell apoptosis rate also significantly increased in OGD group, Conclusion GSRb1, GSRb3 and GSRg1 have protective effects on cultured mouse cortical neurons in OGD in vitro.