水分胁迫对钝顶螺旋藻光合色素和生长的影响

Effects of water stress on photosynthetic pigments and growth of Spirulina platensis

用高效液相色谱和光谱研究了水分胁迫下钝顶螺旋藻的色素变化及复水后的生长状 况．结果表明:藻蓝蛋白对水分胁迫最敏感,其次为叶绿素a和类胡萝卜素;水分胁迫下叶绿素a 含量下降,而类胡萝卜素含量无明显变化,导致类胡萝卜素与叶绿素a的比值升高;复水后藻蓝蛋 白的吸收波段随失水状况呈下降趋势,其荧光发射峰由656 nm蓝移至644 nm;失水率为90％以下 的藻复水后均能逐渐恢复到正常的生长状态;螺旋藻的水分临界值有可能介于70％-80％．

The effects of water stress on photosynthetic pigments and growth of Spirulina platensis were studied. The results show that phycocyanin is most sensitive to water stress, and then are chlorophyll a and carotenoid. The content of chlorophyll a decreases during water stress, while the content of carotenoid has no remarkable change, which lead to the raise of the ratio of Carotenoid/Chl a. The absorption of phycocyanin drops with dehydration, its fluorescence emission peak has blue - shift from 656 nm to 644 nm. Under the water loss rate of 90%, Spirulina platensis are able to retune to normal growth condition gradually after rehydration. The critical water loss rate of Spirulina platensis could be 70% - 80%.