期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Genistein对HIV-1gp120抑制鼠LTP的实验研究

Effect of genistein on long-term potentiation inhibited by gp120 in rat hippocampus in vitro
下载PDF
导出
摘要 目的为了探讨酪氨酸蛋白激酶抑制剂Genistein对人类免疫缺陷病毒Ⅰ型(HIV1)的包膜糖蛋白gp120引起大鼠海马脑片CA1区的长时程增强效应(Longtermpotentiation,LTP)作用的影响。方法应用离体脑片记录技术,记录大鼠海马CA1区的兴奋性突触后电位EPSP,研究了Genistein对gp120引起的大鼠海马脑片CA1区的突触传递和可塑性变化的影响。结果gp120对高频电刺激(HFS,100Hz,1000ms×2,串间隔20s,共2次)Schaffer侧支引起的大鼠海马CA1区LTP产生抑制作用,而对PTP没有影响。酪氨酸蛋白激酶抑制剂Genistein可以反转这种抑制效应。结论gp120可能通过抑制海马CA1区LTP而参与艾滋病痴呆(HIV1associateddementia,HAD)的形成,且这种抑制作用可能与酪氨酸蛋白激酶抑制剂Genistein有关。 Objective To explore the effect of tyrosine kinase inhibitor genistein on long-term potentiation （LTP） inhibited by HIV-1gp120 in the CA1 region of rat hippocampal slices. Methods The excitatory postsynaptic potentials （EPSP） and the effect of genistein on gpl20-induced synaptic transmission and plastic change were recorded and investigated in the CA, region of rat hippocampal slices. Results The results showed that HIV-1 gp120 could inhibit the LTP of CA1, but not the PTP. Protein kinase inhibitor genistein could reverse the inhibitory effect. Conclusion The effect of tyrosine kinase inhibitor genistein on long-term potentiation inhibited by HIV-1gp120 may contribute to the HAD pathogenesis.
作者 董军 谢新华 付咏梅 李传行
机构地区 暨南大学医学院病理生理教研室 中山大学肿瘤防治中心
出处 《免疫学杂志》 CAS CSCD 北大核心 2005年第6期485-488,共4页 Immunological Journal
基金 广东省医学科研基金项目(A2004327) 广东省自然科学基金项目(04010443) 国家中管局择优项目(国中医药科2003LHR13号) 国家中管局三A重点实验室开放课题基金项目(51205026)资助
关键词 人类免疫缺陷病毒Ⅰ型 GENISTEIN GP120 长时程增强 海马脑片 HIV-1 Genistein gp120 Long-term potentiation Hippocampal slices
分类号 R96 [医药卫生—药理学]
  • 相关文献

参考文献13

  • 1de Gans J ,Portegies P .Neurological complications of infection with human immunodeficiency virus type 1.A review of literature and 241 cases[J].Clin Neurol Neurosurg,1989,91 (3):199-219.
  • 2Price RW,Brew BJ.The AIDS dementia complex[J].J Infect Dis,1988,158(5):1079-1083.
  • 3Saito Y,Sharer LR,Epstein LG,et al.Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues [J].Neurology,1994,44(3 Pt 1):474-481.
  • 4Lipton SA,Gendelman HE.Seminars in medicine of the Beth Israel Hospital,Boston.Dementia associated with the acquired immunodeficiency syndrome[J].N Engl J Med ,1995,332(14) :934-940.
  • 5Tornatore C,Chandra R,Berger JR,et al.HIV-1 infection of subcortical astrocytes in the pediatric central nervous system[J].Neurology,1994,44 (3Pt 1) :481-487.
  • 6董军,陆大祥.HIV-1包膜糖蛋白V_3环对大鼠海马长时程增强效应的影响[J].中华微生物学和免疫学杂志,2004,24(3):235-238. 被引量：1
  • 7Meucci O,Fatatis A,Simen AA,et al.Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival [J] .Proc Natl Acad Sci USA,2000,97(14):8075-8080.
  • 8Budd SL,Tenneti L,Lishnak T,et al.Mitochondrial and extramitochondrial apoptotic signaling pathways in cerebrocortical neurons[J] .Proc Natl Acad Sci USA,2000,97(11):6161-6166.
  • 9Kaul M,Lipton SA.Chemokines and activated macrophages in HIV-1 gp120-induced neuronal apoptosis[J] .Proc Natl Acad Sci USA,1999,96(14):8212-8216.
  • 10Ghatan S,Larner S,Kinoshita Y,et al.p38 MAP kinase mediates bax translocation in nitric oxide-induced apoptosis in neurons[J].J Cell Biol,2000,150 (2):335-347.

二级参考文献27

  • 1[1]Lipton S A,Gendelman H E.Dementia associated with the acquired immunodeficiency syndrome[J].N Eng J Med,1995,332(14):934-940.
  • 2[2]Ellis R J,Deutsch R,Heaton R K,et al.Neurocognitive impairment is an independent risk factor for death in HIV infection.San Diego HIV Neurobehavioral Research Center Group[J].Arch Neurol,1997,54(4),416-424.
  • 3[3]Major E O,Rausch D,Marra C,et al.HIV-associated dementia[J].Science,2000,288(5465):440-442.
  • 4[4]Davis L E,Hjelle B L,Miller V E,et al.Early viral brain invasion in iatrogenic human immunodeficiency virus infection[J].Neurology,1992,42(9):1736-1739.
  • 5[5]Meltzer M S,Nakamura M,Hansen B D,et al.Macrophages as susceptible targets for HIV infection,persistent viral reservoirs in tissue,and key immunoregulatory cells that control levels[J].AIDS Res Hum Retroviruses, 1990,6(8):967-971.
  • 6[6]Lanska D J.Epidemiology of human immunodeficiency virus infection and associated neurologic illness[J].Semin Neurol,1999,19(2):105-111.
  • 7[7]Glass J D,Fedor H,Wesselingh S L, et al.Immunocytochemical quantitation of human immunodeficiency virus in the brain:correlations with dementia[J].Ann Neurol,1995,38:755-762.
  • 8[8]Gendelman H E,Persidsky Y,Ghorpade A,et al.The neuropathogenesis of the AIDS dementia complex[J].AIDS,1997,11:S35-45.
  • 9[9]Ensoli F,Fiorelli V,De Cristofaro M,et al.Inflammatory cytokines and HIV-1-associated neurodegeneration:oncostatin-M produced by mononuclear cells from HIV-1-infected individuals induces apoptosis of primary neurons[J].J Immunol,1999,162(10):6268-6277.
  • 10[10]Meucci O,Miller R J. Gp120 induced neurotoxicity in hippocampal pyramidal neuron culture:protective action of TGF-B1[J].Neurosci,1996,16(13):4080-4088.

共引文献1

免疫学杂志
2005年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部