烧伤大鼠肠道组织内Ca^(2+)-ATPase、CGRP含量对胃肠动力功能的影响

Experimental study on relation between changes of Ca^(2+)-ATPase and calcitonin gene related peptide and gastrointestinal motility in scald rats during early postburn stage

目的研究烫伤大鼠早期肠道组织内Ca2+-ATPase、降钙素基因相关肽(calcitoningenerelatedpeptide,CGRP)的变化,探讨该Ca2+-ATPase、CGRP含量变化与肠道推进功能的关系,进一步阐明烧伤后胃肠功能障碍的发生机制。方法选用清洁大鼠30只,采用30%烫伤大鼠模型,随机分为烫伤组、内毒素组及对照组测定烫伤后1h大鼠胃肠推进距离及肠道组织中Ca2+-ATPase和CGRP的含量。结果烫伤及内毒素腹腔注射1h后大鼠肠道碳素墨水推进距离为(53.00±8.88)cm和(91.00±10.22)cm,对照组为(142.00±11.11)cm,两两比较肠道碳素墨水推进距离,烫伤组和内毒素组明显缩短,差异有显著性(P<0.001)。肠道组织内Ca2+-ATPase分别为(263.8±58.30)、(244.4±105.5)和(380.5±116.7)mol/s,烫伤组及内毒素组肠道组织中Ca2+-ATPase与对照组比较,均有不同程度的降低(P<0.05),CGRP的含量分别为(52.38±39.23)ng/mL,(20.48±23.11)ng/mL及(0.75±1.96)ng/mL,烫伤组及内毒素组肠道组织中CGRP含量均有不同程度的增高,两两比较差异有显著性(P<0.01,P<0.05)。结论烫伤早期肠道组织Ca2+-ATPase活性下降及CGRP含量的增高与肠道动力功能的减弱有着十分密切的关系。

[Objective] To investigate the changes of the contents of Ca^2＋-ATPase and calcitonin gene related peptide （CGRP） in intestinal tissue in scald rats during early postbum stage and the relation between the change of CGRP, Ca^2＋-ATPase and gastrointestinal motility, to elucidate the mechanism of intestinal infection. [Methods] Thirty Wistar rats were inflicted by 30% TBSA IIIdegree scalded, were randomly divided into scalded group（10 rats）, endotoxin group （10 rats） and control group （10 rats）. The distance of prepared Chinese ink advance in the small intestine, the contents of Ca^2＋-ATPase and CGRP were measured at one hour post.bum. [Results] The distance of prepared Chinese ink advance in the small intestine was （53.00±8.88） cm in scalded group, （91.00±10.22） cm in endotoxin group, and （142.00±11.11） cm in control group respectively. The distance of prepared Chinese ink advance decreased markedly post.bum in scalded group and in endotoxin group （P 〈0.01）. The contents of Ca^2＋-ATPase was （263.8±58.3） mol/s, （244.4±105.5） molts and （380.5±116.7） mol/s respectively. It decreased remarkably in scalded group and endotoxin group （P 〈0.05）. The contents of CGRP in intestinal tissue of scalded group, endotoxin group and control group was （52.38±39.23） ng/mL, （20.48±23.11） ng/mL and （0.75±1.96） ng/mL respectively, and the differenee was remarkable （P 〈0.01, P 〈0.05）. [Conclusions] The gastrointestinal motility decrease may mainly due to the contents of CGRP in intestinal tissue excessive increase and that of Ca^2＋-ATPase decreased, it may play important roles in the dysfunction of the small intestine during early postbum stage.