摘要
目的:探讨膀胱移行细胞癌中P-糖蛋白(P-gp)、p53、Bcl-2、谷胱甘肽S-转移酶-π(GST-π)的表达,了解多药耐药性与膀胱移行细胞癌细胞凋亡的相关性。方法:采用链霉素-生物素(SP)免疫组化技术检测75例膀胱移行细胞癌和10例无病变膀胱(正常对照组)标本P-gP、p53、GST-π、Bcl-2的表达。结果:膀胱移行细胞癌中P-gP、p53、GST-π、Bcl-2的阳性表达率分别为81.66%、69.33%、80.00%、65.33%;不同病理分级膀胱移行细胞癌中P-gp、GST-π、Bcl-2的阳性表达差异有统计学意义(P<0.05-0.01),不同病理分期膀胱移行细胞癌中P-gP、GST-π、p53、Bcl-2阳性表达差异有统计学意义(P均<0.05);复发病例中P-gP、GST-π表达异常与p53、Bcl-之间存在联系。结论:P-gp、p53、Bcl-2、GST-π等表述与膀胱移行细胞癌的分期有关;P-gP、GST-π高表达在膀胱移行细胞癌中是一种常见现象;耐药指标P-gP、GST-π与凋亡指标p53、Bcl-2影响因素有一定的内在联系。
Objective: This study was designed to investigate the relationship between pathology and P-gp, p53, GST-π, Bcl-2 expression in the grade of differentiation and stage of differentiation of transitional cell carcinoma of the bladder and relationship between multidrug resistance of expression. Methods: Using SP method of immunohistochemical analysis for expression of P-gp, p53, GST-π and Bcl-2. Results: P-gp, p53, GST-π, Bcl-2 protein expression in transitional cell carcinoma of the human bladder from 75 patients showed 82.66%, 69.33%, 80.00%, 65.33% respectively. P-gp, GST-π, p53 and Bcl-2 showed different expression in different stages and grades. Following up expression p53 and Bcl-2, protein expression, P-gp, GST-π affect were obviously changed. Conclusions: p53, Bcl-2 of apoptosis parametres and GST-π, P-gp multidrug resistance of parametres in transitional cell carcinoma of the human bladder, both were obviously associated with the stage of differentiation and the grade of differentiation. Multidrug resistance P-gp, GST-π could affect apoptosis protein expression p53, Bcl-2.
出处
《新疆医科大学学报》
CAS
2005年第9期811-813,共3页
Journal of Xinjiang Medical University
关键词
膀胱肿瘤
移行细胞癌
免疫组化
多药耐药性
bladder neoplasms
transitional cell carcinoma
immunohistochemistry
multidrug resistance