RP-HPLC测定颠茄片中3种主要成分含量

Determination of Three Main Components in Belladonna Tablets by RP-HPLC

目的建立1种测定颠茄片中3种主要成分含量的方法.方法采用反相高效液相色谱法(RP-HPLC)、外标法定量.结果线性关系:阿托品在7.55～37.75μg/ml内良好,山莨菪碱在2.32～20.88μg/ml良好,东莨菪碱在2.5～12.5μg/ml良好.平均回收率:阿托品100.5%(n=3,RSD=1.3%),东莨菪碱99.3%(n=3,RSD=1.6%),山莨菪碱99.9%(n=3,RSD=1.4%).3种莨菪碱在此条件下能完全分离.结论此方法准确、快速分析颠茄片中3种主要成份,灵敏度高,重现性好,药品用量少,可控制颠茄片的质量.

Objective To establish a method for determination of scopolamine , anisodamine and atropine in Belladonna tablets by RP - HPLC. Methods The concentrations were quantified by external standard method and RP-HPLC. Results The calibration curve for atropine , anisodamine and scopolamine revealed linearity over the range of 7.55 - 37.75μg/ml , 2.32 - 20.88 μg/ml and 2.5 - 12.5μg/ml . The average recoveries of atropine, scopolamine and anisodamine were 100.5% （n =3,RSD=1.3 %） ,99.3%（n =3,RSD=1.6 %）,99.9%（n =3,RSD=1.4%） respectively. Conclusion The method is successfully used to rapid assay belladonna teblet with accurate, sensitive, reproducible and little sample needed.