芹灵颗粒抗慢性肝损伤实验研究

Experimental Study of Qin Ling Granule on Chronic Hepatic Injury in Rats

目的观察芹灵颗粒对大鼠慢性肝损伤的治疗作用。方法用CCl4复制慢性肝损伤动物模型(40%浓度,首剂按体重5ml.kg-1,以后按体重3ml.kg-1),3d一次,共42d。ig给药42d,芹灵颗粒3个剂量分别为2、51、0g.kg-1。42d后,取大鼠血和肝脏,测定血清肝功(ALT、AST、ALP、TPR、ALB)及光镜下观察肝脏组织学。结果慢性肝损伤模型组动物:(1)血清TPR、ALB明显降低;(2)ALP、ALT及AST明显升高;(3)病理组织学:肝组织变性坏死显著,肝纤维化明显。芹灵颗粒中、大治疗组可明显改善上述慢性肝损伤诱发的肝功能及组织学变化。结论芹灵颗粒有明显的抗慢性肝损伤及肝纤维化作用。

Aim To investigate the effect of Qin Ling Granule （QL） on chronic hepatic injury in rats. Methods The rat hepatic injury model was induced by the subcutaneous injection of 40% CCl4 （every three day for 42 days）. The QL was given in three groups： 2,5 and 10g· kg^- 1, ig. After 42 treatment days, the blood and liver of rats were taken. The liver function, alanine transaminase （ALT）, alkaline phosphatase （ ALP ）, aspartate aminotransferase （ AST）, total protein （TP） and albumin （ALb） were determined. Histopathological changes were examined by optical microscopy. Results In model group： （ 1 ） The levels of TLP and Alb reduced. （2） The level of AP, ALT and AST increased significantly. （ 3 ） Pathologic histology： Degeneration, necrosis and hepatic fibrosis in liver tissues were seen under optical microscopy. In QL treated groups：all the changes above were improved significantly in middle and high dose treated groups. Conclusion QL possessed the protective effect on chronic hepatic injury and fibrosis induced by CCl4.