胃癌和癌旁黏膜与距癌远端切缘胃黏膜基因表达谱差异的研究

Study on gene expression profile difference in gastric cancer,pericancerous mucosa and normal gastric mucosa from the distant cutting margin by oligonucleotide microarray

目的用基因芯片技术研究胃癌(T)和癌旁黏膜(P)与距癌远端切缘胃黏膜(C)基因表达谱差异,筛选与早期癌变相关的基因。方法利用国际学术界公认的标准化Affymetrix公司生产的U133A基因芯片分别检测T和P及C基因表达谱,并利用生物信息学方法对检测结果进行分析。结果(1)T与C比较差异4倍以上共有766个基因,其中表达上调犤信号比的对数值(SLR)>2犦有530个,表达下调(SLR<-2)有236个;(2)P与C比较差异4倍以上共有64个基因,其中表达上调(SLR>2)有50个,表达下调(SLR<-2)有14个;(3)T和P同时与C比较(T、P两者之一差异4倍以上)共有143个相同的基因,其中表达上调(SLR>2)有108个,表达下调(SLR<-2)有35个。结论癌旁黏膜在基因表达水平上显示143个基因与胃癌表达的基因相同,提示这些基因可能与早期胃癌癌变的启动和演化有关。

Objective To study the difference of gene expression profiles in gastric cancer （T）, pericancerous mucosa （P） and the gastric mucosa from distant cutting margin （C）, and to screen an associated novel gene in early gastric carcinogenesis by oligonucleotide microarray. Methods U133A （Affymetrix, Santa Clara, CA） gene chip was used to detect the gene expression profile difference in T, P and C, respectively. Bioinformatics was used to analyze the detected results. Results When gastric cancer was compared with normal gastric mucosa, 766 genes were found, with a difference of more than four times in expression levels, including 530 up-regulated [Signal Log Ratio （SLR） 〉 2], and 236 down-regulated （SLR 〈- 2） . When P was compared with C, 64 genes were found, with a difference of more than four times in expression levels, including 50 up-regulated （SLR 〉 2）, and 14 down-regulated （SLR 〈-2） . Compared with C, a total of 143 genes with a difference of more than four times in expression levels both in T and P tissues. Of the 143 genes, 108 were up-regulated （SLR 〉 2）, and 35 were down-regulated （SLR 〈- 2） . Conclusions Gene chip can reveal 143 same genes both in pericaneerous mucosa and gastric mucosa. These genes may be related to the carcinogenesis and development of early gastric cancer.