叶绿素A检验对溺死诊断的实验研究

Experimental Studying on Detection of Chlorophyll A for Diagnosis of Drowning

目的量化性研究SD大鼠溺死和死后抛入水中尸体内脏组织中叶绿素A(ChlA,chlorophyll A)检出率。方法应用紫外-可见分光光度计检验单纯脑外伤死亡、脑外伤死后入水和不同水深溺死组大鼠肺、肝、肾及脑内ChlA含量。结果死后入水组,除1例肺内观察到少量ChlA外,其余均未检测出ChlA;溺死组肺内均检测到ChlA,其余脏器部分检测阴性,不同深度溺死组内脏ChlA含量存在差别(P<0.05),溺死组肾组织ChlA含量高于肺组织(P<0.01)。结论检测内脏组织ChlA可为能一种较简便、灵敏的溺死诊断方法。

Objective To study quantitively on detection of chlorophyll A（ChlA） in the viscera! organs of drowning corpse and corpse abandoned in water. Methods Sprague-Dawley rats were divided into five groups：group died of cerebral trauma, group died of cerebral trauma and abandon to water, group drowning in 2 meters,group drowning in 4 meters,group drowning in water surface. Then tile ChlA in the lung, the liver,the kidney and the brain was detected with ultravioletvisible light detector. Results ChlA was detected in lungs of every rats in drowning groups. ChlA was not detected in the tissue of any organ in the control groups. There were significantly different in the quantity of ChlA among the drowning groups（P〈0.05）. The quantity of ChlA in kidneys were greater than that in the lungs in drowning group（P〈0.01）. Conclusion Detection of ChlA may be a simple and sensitive method for the diagnosis of drowning.