原、复发性胶质瘤中VEGF、MMPs表达及其与血管相关超微结构的关系(英文)

Expression of VEGF, MMPs and its relation with vascular ultrastructure in primary and recurrent gliomas

目的探讨原、复发性胶质瘤中血管形成及浸润生长相关因子的表达及意义。方法免疫组化检测67例原发性胶质瘤和14对原、复发性胶质瘤中VEGF、MMP-2和MMP-9的表达,透射电镜观察肿瘤组织中血管相关超微结构。结果VEGF、MMP-2和MMP-9的表达随原发性胶质瘤恶性程度升高而增强,VEGF与MMP-2、MMP-9的表达呈正相关。复发胶质瘤中VEGF、MMP-2和MMP-9的阳性程度较原发肿瘤有所增强。电镜下见血管内皮细胞增生并向基膜外突出,部分基膜有虫蚀样空洞,血管周间隙可见水肿、血浆外渗。结论VEGF、MMP-2和MMP-9在胶质瘤的血管形成和浸润性生长中具有重要作用。

Objective To explore the expression and its implication of angiogenesis and invasiveness related factor in primary and recurrent glioma. Methods Expressions of vascular endothelial growth factor （VEGF）, matrix metalloproteinase-2 （MMP-2） and MMP-9 were detected by immunohistochemical technique. The morphological characteristics of ultrastructure of glioma were observed by transmission electron microscope （TEM）. Results The expressions of VEGF, MMP-2 and MMP-9 varied in different grades of primary glioma. With the elevation of the malignant degree of the primary glioma, positive staining rates of VEGF, MMP-2 and MMP-9 increased significantly. The expression of VEGF correlated with both MMP-2 and MMP-9 expression. Compared with the primary glioma, the immunoreactivities of VEGF, MMP-2 and MMP-9 in recurrent glioma increased, especially in those with more severe malignancy. Under transmission electron microscope, endothelial cells markedly proliferated and protruded from the deficiency of basemembrane, concomitantly with edema of the extracapillary gap, plasma extravasation as well as some small worm-eaten caverns in the basemembrane.Conclusion VEGF, MMP-2 and MMP-9 play important roles in glioma angiogenesis and invasiveness.Inhibition of their expressions may be a useful therapy to glioma.