人胎脑神经干细胞特征性标记物的分布

Distribution of Characteristic Marker of Neural Stem Cells from Human Fatal Brain

目的:探讨人胎脑神经干细胞特征性标记物的分布。方法:收集胎龄16-36周水囊引产胎儿90例,采用免疫组织化学和光镜技术对人胎脑不同部位神经干细胞的特征性标记物分布进行检测。结果:不同胎龄胎脑均存在神经干细胞,Nestin和CD34蛋白双阳性NSCs仅存在于28周胎龄组的海马及室下区、32周胎龄组的海马、室下区及纹状体和36周胎龄组的纹状体,且这类NSCs主要呈圆形和椭圆形,大小不一。Nestin和CD133蛋白双阳性NSCs在不同的胎龄组以及同一胎龄组的不同部位其表达不同,胎龄越小,Nestin和CD133蛋白双阳性NSCs越多,32周胎龄组仅在海马部位发现1个此类NSCs,36周胎龄组的任何部位均未发现此类NSCs。结论:Nestin是人胎脑神经干细胞特征性标记物,CD133和CD34是人胎脑部分神经干细胞特征性标记物。

Objective：To investigate distribution of characteristic marker of neural stem cells （NSCs） from human fatal brain, Methods： Ninety cases of embryos at gestational age of 16-36 weeks and by induction of labor with water bag were collected for determining distribution of characteristic marker of NSCs with SABC immunohis- tochemical method under a light microscope. Results： NSCs existed in different fetal brains at different gestational age. NSCs that had double-expression including Nestin and CD34 from human fetal brain only located in hippocampus and subventicular zone at gestational age of 28 weeks and 32 weeks and in striatum at gestational age of 32 weeks and 36 weeks, which were round and ellipse, large or small. NSCs that had double-expression including Nestin and CD133 had different expression in different gestational age group and different regions at the same gestational age group, The less the gestational age, the more the NSCs that had double-expression including Nestin and CD133. Only one NSC that had double-expression including Nestin and CD133 was discovered in hippocampus at 32 weeks, but none of NSCs were discovered at 36 weeks. Conclusion： Nestin is a characteristic marker of NSCs from human fatal brain. CD133 and CD34 may be the characteristic markers of part of NSCs from human fatal brain.