尿激酶受体在尿激酶诱导小鼠精子趋化运动中的作用

Effects of urokinase-type plasminogen activator receptor on sperm chemotactic response induced by urokinase-type plasminogen activator in mice

目的研究尿激酶受体(uPAR)在尿激酶(uPA)诱导精子趋化运动中的作用。方法采用精子聚集毛细管内法检测精子趋化性。实验分为对照组和处理组,处理组又根据uPAR抗体浓度的不同分为10、50、100μg/ml 3组。各组毛细管内的液体都是浓度相同的uPA;对照组精子培养皿内的液体为精子悬液与Ham’s F-10的混合液,处理组精子培养皿内液体是在对照组的基础上加有不同浓度的抗-uPAR抗体。分别在实验的第203、0 min检测每组毛细管内聚集的精子数量。结果随着uPAR抗体浓度的增加,uPA趋化精子的数目逐渐减少,与对照组之间差异有显著性(P<0.05)。抗-uPAR抗体与精子作用20 min时对精子趋化运动的抑制最明显,其中50、100μg/ml组与对照组比较,P<0.01。结论阻断精子uPAR可以抑制由uPA诱导的精子趋化运动,推测uPAR可能在精卵识别中起一定的作用。

To study the effects of urokinase-type plasminogen activator receptor （uPAR） on sperm chemotactic response （SCR） induced by urokinase-type plasminogen activator （uPA） in mice. Methods. SCR was measured by capillary assay with uPA as the chemoattractant. There were a control group and a treatment group in the experiment. The treatment group was further divided into three sub-groups according to the different concentrations of anti-uPAR antibody added. The fluid in culture wells was a mixture of sperm suspension and Ham＇s F-10, with anti-uPAR antibody added in the treatment group. The sperm accumulation in cap- illary of every group was measured at 20, 30 rain, respectively. Results. 1. Compared with those of the control group, spermatozoa accumulated in capillary of treat group, with the increasing concentrations of anti-uPAR antibody, were decreased obviously （P〈0. 05）. 2. SCR was inhibited most significantly by anti-uPAR antibody at 20 rain. Conclusions： SCR, induced by uPA, could be inhibited by anti-uPAR antibody. It is presumed that uPAR, associated with SCR, plays a role in sperm-egg recognition. A new mechanism of male contraception, that is, inhibiting fertility by interfering with SCR and the sperm-egg recognition, is suggested in this study.