HPLC法测定复方对乙酰氨基酚片中对乙酰氨基酚、咖啡因、乙酰水杨酸的含量

HPLC Determination of Paracetamol Caffeine and Aspirin in Compound Paracetamol Tablets

目的建立一种采用高效液相色谱法同时测定复方对乙酰氨基酚中对乙酰氨基酚、咖啡因、乙酰水杨酸含量的方法〔1〕。方法色谱柱:HypersilODS2(5μm,4.6mm×250mm),流动相:0.02mol·L-1磷酸二氢钾(用磷酸调节pH=3.5)-甲醇(70∶30)〔2〕,UV检测:272nm,流速1.0mL·min-1。结果对乙酰氨基酚、咖啡因、乙酰水杨酸3种成分的回收率分别为99.9%、99.9%、99.7%。RSD分别为0.30%、0.35%、0.68%。结论本法简便、准确,精密度高,重现性好,适用于复方对乙酰氨基酚片的含量测定。

OBJECTIVE To establish a method for determination of paracetamol caffeine and aspirin in compound paracetamol tablets. METHODS HPLC method was carried on a column of Hypersil ODS2 （5μm, 4.6mm× 250mm）,with the mobile phase of methanol-0.02mol·L^-1potassium dihydrogen phosphate solution （pH adjusted to 3.5 by phosphoric acid） and the detection wavelength of 272nm. RESULTS The linear range was 0. 1262mg·mL^-1-0. 6310mg·mL^-1（r=0. 9996 ,n=5）for paracetamal 0. 03024mg·mL^-1-0. 1512mg· mL^-1 （r= 0. 9998,n= 5）for caffeine 0. 2303mg·mL^-1-1. 152mg·mL^-1（r=0. 9996）for aspirin the average recoveries of paracetamal caffeine and aspirin were 99.9% （RSD= 0. 30%, n = 9）, 99. 9% （RSD= 0.35%, n = 9） and 99.7% （RSD=0. 68% ,n=9）. CONCLUSION The method possesses merits of simplicity sensitivity good accuracy high precision and fine reproducibility which is suitable for determination of paracetamol caffeine and aspirin in compound paracetamol tablest.