苦豆碱对松材线虫的杀线活性

NEMATICIDAL ACTIVITY OF ALOPERINE AGAINST PINE WOOD NEMATODES

研究了苦豆草中苦豆碱对松材线虫的杀线活性，讨论了苦豆草生物碱在研究防治松材线虫病中的意义。使用含苦豆碱的培养基培养灰葡萄抱菌菌丝的生物测定方法，测定了苦豆碱对松材线虫的杀线活性，５天后培养基中苦豆碱的ＬＣ５０＝２．６３×１０－５ｇ／ｍｌ，当浓度为１×１０－４ｇ／ｍｌ和２．６３×１０－５ｇ／ｍｌ时，１５天后苦豆碱的杀线率分别为９９．９％和９４．３％。

The strong nematicidal activity of aloperine from Sophora alopecuroides L. against pine wood nematode (Bursaphelenchus xylophilus Stener et Buhrer Nickle) is reported for the first time. The nematicidal activity of aloperine against pine wood nematodes was examined by the bioassy in which the medium containing aloperine was used to culture mycelia of Botrytis cinerea Pers.After 5 days LC= 2. 63 × 10-5g/ml. Moreover, the nematicidal activity of aloperine increased with time. When the levels of aloperine in the bioassy medium were at 1 × 10-4g/ml and 2. 63 × 10-5g/ml,the nematicidal effects after 15 days reached 99. 9% and 94. 3%, respectively. The signifcance of alkaloids from S. alopecuroides in studies on the control of pine wilt desease is discussed.