抗虫转基因欧洲黑杨的Western印迹法分析

WESTERN BLOT ANALYSIS OF TRANSGENIC POPULUS NIGRA PLANTS TRANSFORMED WITH BACILLUS THURINGIENSIS TOXIN GENE

抗虫转基因欧洲黑杨的Ｗｅｓｔｅｒｎ印迹法分析陈颖，李强，李玲，韩一凡，田颖川（中国林业科学研究院林业研究所北京１０００９１）（中国科学院微生物研究所北京１０００８０）关键词Ｂｔ．基因，转基因欧洲黑杨，Ｗｅｓｔｅｒｎ印迹法ＷＥＳＴＥＲＮＢＬＯＴＡＮＡＬ...

Four transgenic Populus nigra plants which were transformed with Bacillus thuringiensis toxin gene and showed high insect resistance were analysed by Western blot method. The result showed that 55kD polypeptides were found in the positive control and four transgenic plants showed Bt. toxin gene was not only inserted into the chromosomes of poplar, but also expressed into protein. The result gave a direct evidence to explain the four plants's high insect resistance.