可溶性磷含量对溶磷真菌培养滤液溶解磷矿粉的影响

Influence of soluble phosphate on rock phosphate solubilization by filtrate of phosphate-solubilizing fungi(PSF)

研究了3种溶磷真菌生长在含有可溶性磷培养基中的培养滤液对宜昌产磷矿粉的溶磷效果,结果表明,菌株P39培养滤液的溶磷活性不受培养基磷浓度的影响,而主要与葡萄糖含量有关,高浓度的碳源供给提高了其培养滤液的溶磷量;菌株P66培养滤液的溶磷活性受到培养基磷浓度和葡萄糖含量的双重影响,在低浓度葡萄糖供给条件下(10 g/L),培养滤液几乎不具有溶磷活性,而在高浓度葡萄糖供给条件下,菌株P66培养滤液溶磷活性大小与培养基可溶性磷含量成正相关;菌株P2.3培养滤液溶磷活性不明显。研究结果证明,溶磷菌的溶磷活性为非诱导效应,这一结论与其它报道结果相反。表5,参10。

The effect of filtrate of 3 isolates of PSF which growth in liquid solution with different content of soluble phosphate on rock phosphate solubilization was reported in this paper. The results showed that no significant influence of medium soluble phosphate on RP solubilization by filtrate of P39 strain, the P-solubilizing activity of filtrate of P39 was mainly dominated by medium glucose content; higher P-solubilizing activity was obtained with higher C source supplement.In contrast with P39, the P-solubilizing activity of filtrate of P66 was affected by both glucose and soluble phosphate content in the medium.That is at low glucose supplement （10 g/L）, almost no P-solubilizing activity was detected in filtrate of P66, but at higher glucose dosage, a positive correlation between the P-solubilizing activity of filtrate of P66 and soluble phosphate content in medium was observed. The filtrate of P2.3 hash＇t found P-solubilizing activity in all treatments. From this experiment, we concluded that the P-solubilizing activity of PSF was not induced by P stress; this conclusion was in reverse with others reports.