经皮下注射树突状细胞治疗膀胱肿瘤的病理改变

Pathological changes of bladder tumor in subcutaneous treatment by sensitized dendritic cells

目的观察肿瘤细胞冻融上清致敏的树突状细胞(DC)治疗膀胱肿瘤(BT)的疗效。方法选取F344大鼠44只,称重后随机分为4组,均采用膀胱灌注N-甲基亚硝基脲(MNU)制成BT;第11周4组分别经皮下注射PBS、未致敏DC、肿瘤抗原及致敏DC,每周1次,共4次;实验第15周,经电子秤、显微镜检测。结果致敏DC组大鼠体重高于其余3组,并与PBS组及未致敏DC组差异明显(P<0.05);致敏DC组膀胱重量低于其余3组且有显著性差异(P<0.05),其余各组间差异无显著性;致敏DC组病理分期明显低于PBS组和未致敏DC组(P<0.05),其余组间差别无统计学意义;致敏DC组病理分级明显低于PBS组(P<0.05),其余组间差别无统计学意义。结论应用致敏DC经皮下注射治疗大鼠膀胱肿瘤可降低肿瘤的病理分期及分级。

Objective To study the therapeutic effects of dendritic cells sensitized by freeze thawing supernatant of bladder tumor cells on bladder tumor in F344 rats. Methods All 44 female F344 rats were irrigated with 0.1 ml 20 mg/ml N-methyl-N-nitrosourea （MNU） into bladders at weeks 0,2,4,6 and 8 for inducing the bladder tumor.They were treated subcutaneously with either 0.1 ml PBS,3.0 × 10^5 unsensitized DC,0.1 ml freeze thawing supernatant of tumor cells,or 3.0 × 10^5 sensitized DC with freeze thawing supernatant of tumor cells respectively at weeks 11,12,13 and 14. At week 15, their bladders were weighed and harvested for observation by naked eye and microscope. Results The weight of bladders in sensitized DC group was （0.15 ± 0.06） g lower than that in PBS group （0.32 ±0.07） g, unsensitized DC group（0.25 ± 0.08） g and freeze thawing supematant of bladder tumor cell group（0.25 ± 0.05 ）g （P 〈 0.05）. The staging and grading of bladder tumor in sensitized DC group were significantly descended compared with PBS group （P 〈 0.05）. Conclusion Sensitized DC subcutaneous injecting can reduce the staging and grading of F344 rats＇ bladder tumor.