新疆维吾尔族血管紧张素转换酶基因多态性与冠心病关系的研究

Relation between ACE gene polymorphism and coronary artery disease in Uighurs of China

目的探讨维吾尔族冠心病血管紧张素转换酶(ACE)基因的多态性分布及多种危险因素与冠心病(CAD)的关系。方法采用两次聚合酶链反应(PCR)方法检测152例维吾尔族包括对照组81例,冠心病组71例,测定各组ACE基因插入/缺失(I/D)多态性,并用微量比色法测定其血清ACE水平。结果与对照组比较,冠心病组DD基因型频率及D等位基因频率高于正常组,但无显著性差异(χ2=2.22,P>0.05)。血清ACE水平冠心病组ID型最高,II型最低,与正常对照组比较无显著性差异(P>0.05)。以性别、年龄、血压、体重指数、血脂指标、ACE水平、瘦素及ACE基因型为变量,行Logistic回归分析冠心病的独立危险因素,显示维吾尔族年龄(OR=1.04)、性别(OR=0.31)、ACE水平(0R=1.05)与冠心病呈正相关;而与血压、体重指数、血脂指标和瘦素水平无关,同时本研究选取的各种基因型均未进入模型。结论ACE基因I/D多态性与维吾尔族人群冠心病的发生无关,DD基因型亦不是维吾尔族冠心病的一个独立危险因素,在CAD的发病中,环境因素可能更重要。

Objective To determine angiotensin-converting enzyme（ACE） gene insertion/deletion（I/D） polymorphism distribution in Xinjiang Uighurs of China and its relation with coronary artery disease（CAD）. Methods ACE genotype was determined by polymerase chain reaction（PCR） ,and serum ACE levels were concomitantly measured by microcolorimetric assay. Of the 152 Uighurs patients, including 71 with CAD and 81 healthy subjects. Results Compared with the healthy control group, the frequency of DD genotype was higher in group CAD, but there was no statistical significance（ χ^2 = 2.22, P 〉0.05）. The serum ACE levels of CAD, ID genotype were higher compared with control group （P 〈 0.05）, but serum ACE levels was no significant difference in other genotypes between two groups（ P 〉0.05）. In Logistic regression analysis, age, sex and ACE levels were positively correlated with CAD, but there were no significant differences in body mass index（BMI）, blood pressure , serum lipids, serum leptin levels, and polymorphism of the genes failed to enter the model. Conclusion The I/D genetic polymorphism of the ACE gene is not associated with the CAD Uighurs population. ACE DD genotype is not an independent risk factor for CAD in the Uighurs population. Environmental factors might be more important than genetic traits in the pathogenesis of essential CAD.