摘要
以5种中国兰花为材料,提取了其叶片基因组DNA;以春兰叶片的基因组DNA为模板,优化了RAPD的反应条件。试验结果表明:春兰基因组DNA的最适扩增条件为,25μL体系,Taq酶0.08U/μL,dNTP240μmol/L,Mg2+1.2mmol/L,随机引物0.5μmol/L,模板DNA6.2ng/μL;反应因子低于最适值,会导致扩增带数减少甚至无带;但若高于最适值,则会出现带的弥散或缺失。
The method for isolating genomic DNA from 5 Cymbidium species was studied. Based on the genomic DNA, some essential factors that affect the result of RAPD were compared and the optimal RAPD system for Cymbidium was established. The results showed that the system with Taq DNA polymerase 0.08 U/μL, dNTP 210 μmol/L, Mg^2＋ 1.2 mmol/L, random primer 0.5 μmol/L, genomic DNA 6.2 ng/μL in 25 μL solution was suitable for Cymbidium amplification reaction.
出处
《热带农业科学》
2005年第4期19-23,共5页
Chinese Journal of Tropical Agriculture
