摘要
目的:测定10个产地蓬子菜中喇叭茶苷和香叶木苷的含量,建立蓬子菜中黄酮类成分含量测定的方法;方法:HPLC法,色谱柱:Hypersil ODS2 5μm(200mm×4.6mm i.d);流动相:甲醇-水-冰醋酸(体积比30:70:0.20);检测波长:340nm.结果:香叶木苷在0.601 0～1.510 μg(r=0.999 9),喇叭茶苷在0.098～0.249 8μg(r=0.996 2)与峰面积线性关系良好.结论:这两个黄酮类成分在10个产地中含量比较稳定,本法简单可行,准确性好,重现性高,是蓬子莱中黄酮类成分含量测定的有效方法.
Objective：To develop an RH - HPLC method for determination of Diosmin and Palustroside in Galium verum L., compare the contents in 10 samples. Methods：Hypersil ODS2 column was used. The mobile phase consisted of methanol - water- glacial acetic acid（30：70：0.20）. Detection wavelengths were set at 340 nm. Results： The linear ranges for Diosmin and Palrstroside were 0. 601 0-1.510μg and 0. 098 - 0. 249 8 μg. Conclusion：This method is sample, rapid and accurate, and can be used for the determination of Diosmin and Palustroside in C, alium verum L.
出处
《中国野生植物资源》
2005年第5期62-64,共3页
Chinese Wild Plant Resources
基金
黑龙江省攻关计划(2003)GC03C402-1
