药用植物茅苍术的组织培养

Tissue Culture of Atractylodes lancea(Thunb.) DC.

目的针对茅苍术野生资源濒危的情况,系统地探讨了以组织培养为手段进行快速繁殖的途径。方法以叶柄和叶片为外植体,在MS培养基上添加不同的激素配比进行培养。结果较适宜诱导叶柄形成愈伤组织的激素组合是2,4-D 4.0 mg.L-1+Kt 0.4 mg.L-1,叶片适宜诱导愈伤组织的激素组合是2,4-D4.0 mg.L-1+Kt 0.4 mg.L-1;对于芽的增殖,较适宜的激素组合是6-BA2.0mg.L-1+IBA0.4 mg.L-1或6-BA3.0 mg.L-1+IBA0.4mg.L-1;而根的诱导则在MS+IAA0.5 mg.L-1或MS+NAA0.5 mg.L-1培养基上进行最好。结论采用组织培养方式可以进行茅苍术的快速繁殖,为确保这一珍稀药用植物资源的保护和可持续利用提供了有效途径。

Objective To research on the methods of rapid propagation of Atractylodes lancea （Thunb.） DC. by way of tissue culture. Methods The petiole and the leave were chosen the best material explants in rapid propagation. These explants were cultured on MS culture media by adding different portion of hormones in various cultural conditions. Results For the petioles , the suitable hormone combination to induce callus is 2,4-D 4.0 mg · L^-1＋Kt 0.4 mg ·L^-1 ;for the leaves ,it is 2,4-D 4. 0mg · L^-1＋Kt 0. 4 mg · L^-1;for the adventitious buds ,they are 6-BA2.0 mg · L^-1 ＋IBA0.4 mg · L^-1or 6-BA3.0 mg · L^-1＋IBA0.4 mg · L^-1 ;and for the roots , they are MS＋IAA0.5 mg · L^-1 or MS＋NAA0. 5 mg · L^-1 , respectively. Conclusion To preserve resources and have a steady utilization , it provided effective way for tissue culture of A. lancea（Thunb. ） DC.