血管内皮生长因子受体KDR在子宫内膜异位症中的表达

Expression of vascular endothelial growth factor receptor-kinase insertion domain-containing receptor in endometriosis and human normal endometrium Nimazhuoma

目的探讨血管内皮生长因子受体(VEGFR)胎肝激酶(KDR/flk-1)在子宫内膜异位症(内异症)患者中异位、在位子宫内膜及正常子宫内膜组织中的表达及意义。方法采用半定量RT-PCR(逆转录聚合酶链反应)和免疫组化(SABC法)方法,从基因和蛋白质水平检测44例内异症患者子宫内膜组织(卵巢子宫内膜囊肿32份)及30份在位内膜组织(研究组)中VEGFRKDR的阳性表达率。选择40例非内异症患者的正常子宫内膜组织作为对照组。结果两组多数异位及在位子宫内膜组织中均可检测KDR的表达,内异症组在位内膜和异位内膜KDR相对表达强度明显高于对照组,差异有统计学意义(P<0.05);不同月经周期KDR的表达,在增殖期KDR相对表达强度高于分泌期,差异有统计学意义(P<0.05)。结论VEGF受体KDR在内异症在位内膜和异位内膜中有明显表达,可能VEGF受体参与内异症血管生成,促使异位病灶远处转移种植、浸润;在位内膜增殖期KDR相对表达强度高于分泌期可能与卵巢激素相关。

Objective To study the expression of vascular endothelial growth factor receptor- KDR（kinase insertion domain- containing receptor） in ectopic and eutopic endometria of patients with endometriosis and normal endometrium.Methods Thirty - two specimens of ovarian endometrioma and 30 specimens of matched eutopic endometrium were biopsied from 44 patients with endometriosis; another 40 speciments of endometrium were biopsied from women without endometriosis. Reverse transcription- polymerase chain reaction（RT- PCR） and immunohistochemistry（streptavidin- biotin complex, SABC） analysis were utilized to examine KDR mRNA and protein expression and their levels in the above- mentioned endometria.Results The KDR levels in ectopic and eutopic endometrium of patients with endomefriosis were significantly higher than that in normal controh （P 〈0.05）. The expression of KDR in the proliferative phase endometrium was significantly higher than that in the secretory phase endometrium（P 〈0.05）. Conclusions The VEGF receptor- KDR is highly expressed in both the ectopic and eutopic endometria of women with endometriosis, indicating that VEGF receptor may be involved in endometriotic angiogenesis, and promotes remote metastasis, implantation and infiltration. Higher expression of KDR in proliferative phase eutopic endometrium than that in secretory phase endometrium may be related to hormone function.