膀胱移行细胞癌组织中PTEN及VEGF的表达及相互关系

Expression and relationship between PTEN and VEGF in bladder transitional cell carcinoma

目的探讨第10号染色体丢失的磷酸酶基因(PTEN)和血管内皮生长因子(VEGF)在膀胱移行细胞癌组织中的表达及其相互关系和临床意义。方法采用免疫组化SP法观察60例膀胱移行细胞癌石蜡标本中PTEN和VEGF的表达情况。结果28例(47%)呈PTEN蛋白阳性表达,41例(68%)呈VEGF阳性表达。随着病理分级、临床分期的增高,PTEN的阳性率降低(P<0.05);VEGF的阳性率相应增加但无统计学意义(P>0.05),但转移组和未转移组间有显著性差异(P<0.05);PTEN与VEGF两者之间呈明显负相关关系(r=-0.439,P<0.01)。结论膀胱癌组织中PTEN和VEGF的表达在肿瘤进展过程中起重要作用并有助于预后判断。

Objective To investigate the expression and relationship between phosphatase and tensin homology deleted on chromosome ten （PTEN） and vascular endothelial growth factor （VEGF） in bladder transitional cell carcinoma （BTCC） and their clinical significance. Methods Expression of PTEN and VEGF were detected in 60 specimens by immunohistochemistry test （SP method）. Results The positive rate of PTEN was 47%（28/60） in BTCC; VEGF was 68%（41/60）. With the pathological grade and the clinical stage of tumors being higher, the lower expression level of PTEN showed （P 〈0.05） ; while the expression of VEGF increased with no statistical significance （ P 〉0.05）, but having a significant difference between specimens with lymph nodes metastasis and without lymph nodes metastasis （ P〈0.05）. The expression of PTEN was negatively correlated with that of VEGF （ r = -0. 439, P〈0.01）. Conclusion The expression of PTEN and VEGF in BTCC plays an important role during the progress of carcinoma and is helpful to evaluate the prognosis of patients.