Human AnnexinⅤ在不同胎盘中的表达的定量分析

Quantification and expression of human annexin Ⅴ in kinds of placenta

目的观察人膜联蛋白Ⅴ(Human AnnexinⅤ,HAⅤ)在乙肝病毒感染胎盘、正常中、晚孕胎盘及早孕绒毛中的表达并探讨其意义。方法用SP法检测HBsAg阳性孕妇足月分娩胎盘、HBV血清标志物阴性的正常早孕、中孕和足月胎盘各20例HAⅤ的表达、分布并进行定量分析。结果中孕、足月胎盘和HBV感染的胎盘组织中HAⅤ呈不均一的棕黄色颗粒状染色,泛存在于胎盘组织各类细胞胞浆、胞膜和核膜上。滋养细胞呈强阳性染色,绒毛间质细胞和毛细血管内皮细胞呈弱阳性染色。早孕绒毛HAⅤ棕黄色颗粒主要分布于外层滋养细胞的胞浆、胞膜和核膜上,最内层滋养细胞和间质偶见表达;比较HBV感染组和正常足月组胎盘HAⅤ表达的量,前者的平均灰度比后者明显增高,差异具有显著性(P<0.01),中孕组与足月组HAⅤ表达的量没有差别(P>0.05),早孕和中孕组、足月组HAⅤ表达的量的比较,前者显著低于后两者(P<0.05)。结论HAⅤ可能作为受体介导了HBV对胎盘的感染。

Objective： To investigate the relationship between expression of Human Annexin Ⅴ （HA Ⅴ ） and HBV infection. Methods： SP immunohistechemistry was used to observe the distribution of HAⅤ in the placenta from HBsAg sempositive and term gravida. And the difference of expression of HAⅤ between HBsAg positive group and HBsAg negative group and among tmphoblast cell in different period of gestational age was investigated. Results. HAⅤ existed in cytoplasm, plasmalemma and nuclear membrane of trophoblast cells, villus stroma cells, villus capillary endothelium cells in every sample except early pregnant placenta. Strong expression signal was detected in cytoplasm, plasmalemma and nuclear membrane of tmphoblast cells and weak signal was found in villus stmma cells, villus capillary endothelium cells. On early pregnant villus, HAⅤ mainly distributed in outer tmphoblast cells , there was nearly no positive signal detected in inner tmphoblast cells and villus stroma cells. The concentration of HAⅤ expression in HBV infected placenta was significantly higher than normal placenta（ P 〈 0.01）, HAⅤ showed no statistical difference between middle pregnant placenta and term pregnant placenta（ P 〉 0.05）, however, HAⅤ expression in early pregnant villus was significantly lower than middle pregnant placenta and term pregnant placenta（ P 〈 0.05）. Conclusion： HAⅤ may serve as a hepatitis B Viral receptor to cause HBV infect placenta.