孕中期妇女血清AFP、F-βhCG测定筛查唐氏综合征

Screening for Down syndrome by detecting serum AFP and F-β hCG in pregnant women during second-trimester

目的探讨检测孕中期妇女(14～20w)外周血清中甲胎蛋白(AFP)的游离绒毛膜促性腺激素(F-βhCG)的含量对于筛查唐氏综合征患儿的有效性.方法应用磁性分离酶免疫测定技术测定血清中AP、F-βhCG的含量,结合孕妇临床资料用分析软件进行分析,并通过羊水细胞染色体核型分析给予确诊.结果在接受血清筛查的2849例孕妇中,筛出唐氏综合征高危312例,筛查阳性率为10.95%,其中有174例接受羊水染色体检查,发现2例唐氏综合征患儿.筛出神经管缺损高危61例,全部做了B超检查,发现2例神经管缺损患儿.结论测定孕中期妇女血清AFP、F-βhCG的含量并进行风险分析,对筛查唐氏综合征患儿,降低其出生机率具有重要意义.

Objective：To evaluate the feasibility of screening for Down syndrome by detecting serum AFP and F- β3hCG in pregnant women during second - trimester. Methods： Magnetic - separated enzyme immunoassay technology was used to detect serum AFP and F - βhCG, then the data and clinical materials of pregnant women were analysed by special software.Then chromosomal karyotype analysis on amniotic fluid cell was used to make a further diagnosis. Results：2849 cases of pregnant women＇ s serum were detected.312 cases were found with fetuses at high risk of Down syndrome, the positive rate is 10.95 % ;the fetuses of 2 cases were diagnosed as Down syndrome in 174 cases detected by chromosomal karyotype analysis on arnniotic fluid cell.61 cases were found with fetuses at high risk of Neural Tube Defects （NH））, the fetuses of 2 cases were diagnosed as NTD by B - ultrasonography. Conclusion： Risk assessment of Down syndrome by detecting scrum AFP and F -βhCG in pregnant women during second - trimester is important in screening and decreasing the birth rate of Down syndrome.