大肠癌组织COX-2的表达及其与肿瘤细胞增生和凋亡的关系

The expression of COX-2 and its relationship to the apoptosis and proliferation in colorectal carcinoma

目的探讨大肠癌中环氧化酶-2(cyclooxygenase-2,COX-2)表达及其与癌细胞增生和凋亡的关系。方法采用免疫组织化学法检测60例大肠癌、20例大肠腺瘤和20例癌旁正常大肠黏膜中COX-2和PCNA的表达,并采用TUNEL法检测原位细胞凋亡水平。结果大肠癌组织中COX-2阳性表达、PI及AI均显著高于大肠腺瘤及正常大肠黏膜组织(P<0.05);COX-2的高表达与大肠癌肿瘤大小、淋巴结转移和临床分期有关,与肿瘤生长部位、分化程度无关。COX-2阳性大肠癌组中PI高于COX-2阴性大肠癌组,AI低于COX-2阴性大肠癌组(P<0.05)。结论COX-2的过度表达可促进大肠癌细胞增生、抑制细胞凋亡,在大肠癌的发生、发展过程中起着重要的作用。

Objective To investigate the expression level of cyclooxygenase-2 （COX-2） and its effects on cell proliferation activity and apoptosis in colorectal carcinoma. Methods The expression of COX- 2, proliferating cell nuclear antigen （PCNA） and apoptosis in 60 cases of colorectal carcinoma, 20 cases of colorectal adenoma and 20 cases of normal mucous were studied by the immunohistochemical and TUNEL methods, the apoptosis index（AI）and proliferation index（Pl） were defined respectively. Results The positive expression rate of COX-2 in colorectal carcinoma was significantly higher than that in the other groups. Overexpression of COX-2 in colorectal carcinoma was related to its lymph node metastasis and the size of tumor and clinical stages. In the group with COX-2 positive expression, PI was higher than that in the group with COX-2 negative expression; whereas AI was higher in the latter group than in the former group. Conclusions The expression level of COX-2 may have a close correlation with cell proliferation and apop- tosis, they may participate in oncogenesis and progression of colorectal carcinoma.