葡萄多酚含量和多酚氧化酶(PPO)活性与组培苗生根关系的初步研究

The Effect of Polyphenol Content and Polyphenol Oxidase Activity on Rooting of Tissue Cultured Seedlings in Vitis L.

研究测定了葡萄属10个种(品种)组培苗的多酚含量和多酚氧化酶(PolyphenolOxidase,PPO)的活性,对其与组培苗的生根指数进行相关性分析,并建立生根指数与多酚含量、多酚氧化酶的多项式回归方程。结果表明:不同种(品种)间多酚含量和PPO活性有显著性差异;多酚含量和PPO活性相关性显著,PPO活性和生根指数不相关,而多酚含量与生根指数呈显著负相关;从回归方程可以看出,PPO活性对生根指数的作用是非线性的,多酚含量对生根指数的作用是线性的,这说明多酚含量对组培苗生根的直接作用很大,PPO活性通过多酚含量间接影响组培苗生根而且直接作用较小。

Polyphenol content and Polyphenol Oxidase （PPO）activity of tissue cultured seedlings in Vitis L. were detected, the correlation with rooting was analyzed, and the polynomial regression model was optimized. The result showed that there was significant difference in different species/varieties,but no correlation between PPO activity and rooting index, while phenol content had negative correlation relationship with rooting index, which suggested more phenol may increase difficulty in rooting. Effect of PPO activity on the rooting index is non linear, but polyphenol content has a significant effect on the rooting, while the PPO activities have few significant effect on the rooting, and influence the rooting through the polyphenol contents indirectly.