摘要
1.比较了在卵母细胞成熟液中添加相同浓度(10ng/ml)的表皮生长因子(EGF),胰岛素样生长因子(IGF),EGF+IGF后,不同生长因子对卵母细胞体外发育率的影响。结果表明,在成熟培养液中添加EGF后,其卵母细胞的成熟率(70.9%)与对照组(67.3%)相比有所提高,但差异不显著;在成熟培养液中分别添加IGF、EGF+IGF,其卵母细胞的成熟率分别为80.0%,82.4%,显著高于对照组卵母细胞的成熟率(67.3%)。2.比较了不同季节采集的卵母细胞在体外成熟的情况。结果发现,12月份到来年的1月份,采集的卵母细胞,其体外成熟率最高,为81.0%;11月份采集的卵母细胞,其体外成熟率次之,为74.1%;3—5月份和9—10月份采集的卵母细胞,其体外成熟率为66.0%、69.6%;6—7月初采集的卵母细胞,其体外成熟率最差,为54.7%。3.比较了OM液pH值对卵母细胞体外成熟的影响。当OM培养液的pH值为7.2和6.8￣7.0时,卵母细胞成熟率的为66.2%和61.0%,显著高于pH≥7.5时的成熟率(50.9%,P<0.05)。
Goat oocytes were cultured in maturing media （OM） adding equal concentrations （10 ng/ml） EGF, IGF, or EGF＋IGF. The matured rate （80.0%, 82.4%） of goat oocytes in OM adding IGF and 10 ng/ ml （EGF＋IGF） were higher significantly than in OM and OM adding EGF（70.9%, 67.3%）. Senson can influence the matured rate of goat oocytes. The study show, from December to next year January, that the matured rate of goat oocytes is the highest （81.0%）; the matured rate of goat oocytes is 74.1% at November; the matured rate of goat oocytes is the lowest （54.7%） from June to July. pH of OM media can influence the matured rate of goat oocytes. When pH of OM is 6.8-7.0 and 7.2, the matured rate of goat oocytes were higher significantly than that pH of OM is alkaline （≥7.5）.
出处
《中国农学通报》
CSCD
2005年第9期1-3,16,共4页
Chinese Agricultural Science Bulletin
基金
国家(863)高技术研究与发展计划项目"家畜体细胞克隆技术体系的建立和应用"(2001AA213081)
