CD105和Ⅷ因子相关抗原对大肠癌新生血管标记的研究

Studies on the Expression of CD105 and Factor Ⅷ Related Antigen(F-8 RAg) in Angiogenesis of Colorectal Cancer

目的探讨CD105和Ⅷ因子相关抗原(F-8RAg)在大肠癌的表达意义,并将二者对大肠癌新生血管的标记作用进行对比。方法采用鼠抗人DC105单克隆抗体和鼠抗人第Ⅷ因子相关抗原单克隆抗体,应用免疫组化技术对40例大肠癌手术切除的新鲜标本及40例正常对照组织的微血管进行定量检测,计数100倍视野下5个血管丰富视野的微血管密度(MVD),取其均值。结果大肠癌组织与正常对照组织的微血管密度(MVD)间的差异具有非常显著性(P<0.01);以DC105和Ⅷ因子相关抗原为新生血管标记物测得的大肠癌微血管密度间的差异具有非常显著性(P<0.01);以DC105标记的大肠癌微血管密度与大肠癌的淋巴结转移、远处转移及临床分期有关(P<0.01);而以Ⅷ因子相关抗原为标记物测得的MVD与淋巴结转移、远处转移及临床分期无关(P>0.05)。结论大肠癌新生血管形成与肿瘤的生长、转移及预后有关,DC105和Ⅷ因子相关抗原均在大肠癌有良好表达,但CD105在标染肿瘤新生血管上比因子更具特异性。

Objective：To determine the expression of CD105 and F-8 RAg in colorectal cancer, and make a comparison between them in the marking of colorectal neoplasm-related angiogenesis（NRA）. Methods ：40 cases of colorectal cancer tissue and 40 cases of normal control tissue were investigated by immunohistochemical staining with anti-CD105 monoclonal antibody and anti-F-8 RAg monoclonal antibody. Results： The microvessel density （MVD） marked by CD105 and F-8 RAg was found to be significantly higher in colorectal cancer tissue than in the normal control tissue （ P 〈0. 01）. MVD of colorectal cancer stained by CD105 was significantly higher than that stained by F-8 RAg（ P 〈0.01）. The MVD stained by CD105 was closely associated with lymph node metastasis, distant metastasis and Dukes stages （ P 〈0.01）, but the MVD stained by F-8 RAg didn＇t have the relations with them. Conclusion： Angiogenesis is associated with growth, metastasis and prognosis of colorectal cancer. Both CD105 and F- 8 RAg have good expression in colorectal cancer, but CD105 is better than F-8 RAg in staining neoplasm related microvessels.