人参和泼尼松对关木通致大鼠急性肾毒性的干预作用

Intervention effect of ginseng and prednisone on aristolochia manshuriensis induced acute renal toxicity in rats

目的:探讨中药人参和泼尼松对关木通所致急性肾毒性的干预作用。方法:54只清洁级雌性SD大鼠随机分组,以关木通煎剂40 g.kg-1.d-1制备急性肾毒性模型。分别给予泼尼松(30 m g.k-g 1.-d 1)和生晒参煎剂(40 g.k-g 1.-d 1)灌胃5 d;第4和8 d测定血、尿生化等指标,免疫组化染色进行肾组织病理学观察;用SPSS11.5软件进行统计学处理。结果:含关木通各组大鼠体重和活动能力下降,而血尿素氮(BUN)和肌酐(C r)、尿N乙酰葡糖胺(NAG)酶、尿2β微球蛋白(2βM G)、转化生长因子β1(TGFβ1)、Ⅳ型胶原均有不同程度增高,其中关木通组差异最大;与单纯关木通组比较,各实验组BUN、C r、尿NAG酶、TGFβ1、Ⅳ型胶原有不同程度的降低。结论:单独用人参或泼尼松与关木通混合灌胃,或同时用人参、泼尼松和关木通混合灌胃,均能减轻关木通对大鼠的急性肾毒性。

Objective： To investigate the intervention effect of ginseng and predinsone on aristolochia manshuriensis -induced acute renal toxicity in rats. Methods： Fifty -four cleaning grade female Sprague- Dawley （SD） rats were randomly divided into five groups. Animal models of acute renal toxicity were established by 40g·kg^-1·d^-1 of aristolochia manshuriensis decoction via gastric tube. Prednisone （30mg·kg^-1·d^-1） and dried raw ginseng decoction （40 g·kg^-1·d^-1） were given via gastric tube for 5 days. On the 4 th and 8 th days after administrating the drug, biochemical indexes of the rats＇ blood and urine were detected, and immunohistochemical technique was used to estimate the expressions of transforming growth factor-β1 （TGF-β1） and type Ⅳ collagen in the renal tissue of rats. The pathologic changes of kidney were also observed by microscopy. The SPSS 11.5 packages were used to analyze the data. Results： The rats＇ weight and activity decreased significantly but the levels of blood urea nitrogen （BUN）, creatinine （Cr）, urine N - acetylglucosaminidase （NAG）, urine β2 - microglobulin （β2 - MG）, transforming growth factor- β1 （TGF- β1） and collagen-Ⅳ increased significantly in the groups administered with aristolochia manshuriensis, especially in the aristolochia manshuriensis group. The changes of BUN, Cr, urine NAG, TGF - β1 and collagen Ⅳ were attenuated after feeding the rats with prednisone and ginseng compared to those of the rats administered with aristolochia manshuriensis alone. Conclusion： Aristolochia manshuriensisinduced acute renal toxicity in rats could be relieved by ginseng or prednisone alone or combined.