ELISA法快速检测慢性肝病患者血清中TIMP-1

ELISA for rapid detection of serum TIMP-1 in patients with chronic liver diseases

目的应用酶联免疫吸附方法(ELISA)快速检测慢性肝病患者血清中基质金属蛋白酶组织抑制-剂1(TIMP-1),了解TIMP-1血清学检测与肝纤维化病程进展程度。方法以抗人TIMP-1单克隆抗体为包被抗体,15%小牛血清为封闭液,辣根过氧化物酶(HRP)标记的羊抗人IgG作为酶结合物建立检测人血清TIMP-1 ELISA法,用于临床检测399例肝病患者血清和健康人血清105例。结果临床检测表明肝硬化患者TIMP-1检出阳性率为76.9%,明显高于慢性肝炎(40.7%)和急性肝炎(19.4%)(P<0.005),并随着纤维化程度加重而升高(P<0.01)。结论血清中TIMP-1的变化可作为肝纤维化较为有用的诊断指标。ELISA法具有较高的敏感性、特异性和快速性,试剂用量少,且不需复杂的仪器设备,加之实验程序简单,甚适于医院及基层医疗单位应用。

Objective Using ELISA for rapid detection of TIMP-1 in serum in patients with chronic liver diseases. Methods Preeoating micmplate with anti-TIMP-1 MeAb, confining liquid with 15% calf serum, and sheep anti-httman IgG with HRP were used in the detection of 399 patients. Results The positive rate of the liver cirrhosis group was 76.9 %, significandy higher than in the groups of the ehronie hepatitis （40.7 % ） and the acute hepatitis （ 19.4 % ） （ P 〈 0.005）. Conclusion The serum TIMP-1 level is very useful in the assessment of liver fibrosis in chronic liver disease. ELISA is an ideal technique for rapid detection of TIMP- 1 from the serum.