摘要
支气管败血波氏杆菌是家兔鼻炎和支气管性肺炎的主要病原之一,感染率可达20～70％,严重影响养兔业的发展。该病的诊断通常采用病原分离鉴定,虽具有准确可靠的优点,但费时又费力。
Dot-ELISA detecting antibodies against rabbit Bordetella brochiseptica was established. The resit demonstrated that Dot-ELISA was sensitive,specific and fast Its sensitivity was 260 ~ 320 times higher than microagglutination test (MAT).The detective rate was 9. 5% higher than isolation of B. brochiseptica data of the maternal antibody and vaccinated rabbit antibody impiyed that Primary immunization for younger rabbits should be taken on 30 days old,and followed by the boost after 5~6 months.
