尼维地平降压治疗对大鼠局灶脑缺血的保护作用

Effects of antihypertensive therapy with nilvadipine

选用12周的自发性高血压大鼠,皮下植入渗透压泵持续注入尼维地平成其溶剂,第1、2组分别用药7天和14天,并分别于用药后第6天和第13天阻断左侧大脑中动脉。局灶脑缺血24小时后行病理检查。应用尼维地平治疗后血压降低,冠状连续切片上,第1组有2张、第2组有5张梗塞灶缩小。因此,尼维地平对自发性高血压大鼠的局灶脑缺血有保护作用,且依赖其疗程,提示长期应用此药抗高血压治疗,可减轻脑卒中的病情。

Mice, 12 weeks old, were given either nilvadipine (3 mg/kg/day) or a vehicle subcutaneously. Group 1 was treated for 7 days, and group 2 for 14 days. The left middle cerebral artery was occluded on the 6th (group 1) or 13 th (group 2) day of the treatment, and neurological and neuropathological results were quantified 24 h later. The mean arterial blood pressure was reduced to normal levels with use of nilvadipine. The neurological findings of groups 1 and 2 were better than those of the control group and the infarct volumes of groups 1 and 2 were less than those of selfcontrols. On 8 coronal sections, the infarct size was found to decrease in the 5 sections in group 2, but only in 2 sections of group 1. A significant decrease in the edema volumes was observed in group 2, but not in group 1. The results proved that nilvadipine possesses protective effects against cerebral ischemia in rats with chronic hypertension, and the effects were dependent on the duration of therapy. It is suggested that this anti-hypertensive drug may have additional benefit of reducing the consequences of a possible later stroke in hypertensive patients under long-term care.