摘要
对核桃RAPD分析中M g2+、引物、dNTPs、T aqDNA聚合酶浓度及复性温度等因素进行了比较影响分析。建立了适合核桃DNA多态性分析的RAPD反应体系:25μL反应液中,3.5 mMM g2+、0.2 mM dNTPs、0.2μM随机引物、1.0U T aq酶、50 ng^100 ng模板DNA;反应过程为45个循环,94℃变性60 s,36℃退火60 s,72℃延伸120 s。
The better RAPD reaction system were structured by studying the factors affecting the walnut RAPD reaction. The system contained 3.5 mM MgCl2,0.2mM dNTPs,0.2uM（each） primer, 1.0U Taq DNA polymerase,50 ng-100 ng DNA template in 25ul. PCR amplification was for 45 cycles of 94℃ for 60s,36℃ for 60s,and 72℃ for 120s.
出处
《山西林业科技》
2005年第3期20-22,共3页
Shanxi Forestry Science and Technology
基金
山西省青年基金项目(20021047)
关键词
核桃
RAPD
优化
walnut
RAPD
reaction system
