大蒜鳞芽生长点离体培养诱导小鳞茎的形成

Garlic Bulblet Formation Via the in Vitro Culture of the Bulbous Apical Meristem Scaly Bud

用大蒜鳞芽生长点进行离体培养，结果表明，生长素ＮＡＡ浓度在０．１～１．０ｍｇ／１之间，细胞分裂素６—ＢＡ浓度在０～０．５ｍｇ／１范围内，其浓度愈低愈能促进小鳞茎的形成；贮期温度愈低，愈有利于小鳞茎的形成；３～８℃低温期大于６０天，才能保证小鳞茎的形成。

The scaly bud apical meristem culture was studied using s local garlic cultivars and the results were as follows:on the Ms medium with NAA concentration between 0. 1～1. 0 mg/1 and 6-BA 0￣0. 5mg/1,the lower NAA and 6-BA medium resulted in better bulblet formation and the low temperature of storage life also favor the bulblet formation,which was induced only under the temperature between 3￣8℃ for more than 60 days.